Regulatory

Part:BBa_K4244037

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


Medium promoter + medium RBS

The medium promoter from Wenk et al. was combined with an RBS derived from RBS calculator
In between these a target region was introduced including a PAM and the t5 protospacer, which is targeted by dSpyCas9.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Wenk, S., Yishai, O., Lindner, S. N., & Bar-Even, A. (2018). An Engineering Approach for Rewiring Microbial Metabolism. In Methods in Enzymology (Vol. 608, pp. 329–367). Academic Press Inc. https://doi.org/10.1016/bs.mie.2018.04.026

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